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This study was carried out to investigate the antifungal activities of the extracts from various parts of three Pious species, P. densiflora, P. rigida and P. koraiensis to pathogenic fungus Colletotrichum gloeosporioides. The EtOAc fraction from the bark of P. koraiensis stem and root showed 98.8 and 100% of activity, respectively to the fungus. Median effective doses (ED_(50)) of above two fractions were 469 and 588 ㎍/㎖, respectively in the bioassay with the fungus. ED_(50) of the EtOAc fraction from the bark of P. koraiensis stem against Alternaria brassicicola and Fusarium oxysporum was 533 and 2,277 ㎍/㎖, respectively. This means that the fraction was more sensitive to the C. gloeosporioides and A. brassicicola than the fungus F. oxysporum. The EtOAc fraction from the leaves of P. densiflora showed 39.6% of activity to C. gloeosporioides, but all the fractions from the leaves of two species showed no activity. The active compounds in the bark of P. koraiensis stem and root are being identified.
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